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While it was to remain under Chinese sovereignty, the Portuguese came to consider and administer it as a de facto colony.

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They erected a stone marker at Lintin Island claiming it for the King of Portugal, Manuel I.

In the same year, the Indian Viceroy Afonso de Albuquerque commissioned Rafael Perestrello — a cousin of Christopher Columbus to sail to China in order to open up trade relations.

In 1557, the Ming court finally gave consent for a permanent and official Portuguese trade base at Macau.

In 1558, Leonel de Sousa became the second Portuguese Governor of Macau.

Rafael traded with the Chinese merchants in Guangzhou in that year and in 1516, but was not allowed to move further.

Portugal's king Manuel I in 1517 commissioned a diplomatic and trade mission to Guangzhou headed by Tomé Pires and Fernão Pires de Andrade.They were able to defend their settlements and establish themselves there.Mong Há has long been the center of Chinese life in Macau and the site of what may be the region's oldest temple, a shrine devoted to the Buddhist Guanyin (Goddess of Mercy).Later in the Ming Dynasty (1368–1644 AD), fishermen migrated to Macau from various parts of Guangdong and Fujian provinces and built the A-Ma Temple where they prayed for safety on the sea.The Hoklo Boat people were the first to show interest in Macau as a trading centre for the southern provinces.The embassy lasted until the death of the Zhengde Emperor in Nanjing.