The use of this herb has also been translated in clinics to treat herpes infection in Thailand .
The cholophyll derivatives (phaeophytins) of CN chloroform extracts contained 13-R)-phaeophytin and exhibited antiherpes simplex activity , potentially through herpes virus inactivation and inhibition preinfection .
Galvinoxyl radical scavenging activity of each extract was determined by electron spin resonance spectrometry (JEOL-JES-FA100, Tokyo, Japan) using the following parameters: sweeping field, m T; microwave power, 4 m W; modulation width, 0.2 m T; sweep time, 30 s; time constant, 0.1 s; and amplitude, 120.
nutans on cancer cell lines.1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, galvinoxyl radical, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), phosphate buffer saline (PBS), trypan blue, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and 10x trypsin-EDTA solution were purchased from Sigma Chemical Co. Hydrogen peroxide and sodium nitroprusside (SNP) were purchased from Merck, Malaysia. nutans leaves were freshly harvested and oven-dried at 40–45°C.All the cancer cell lines, including human liver hepatocellular carcinoma (Hep G2), human neuroblastoma cell line (IMR-32), human lung cancer cell line (NCI-H23), human gastric cancer cell line (SNU-1), human colon adenocarcinoma cell line (LS-174T), human erythroleukemia cell line (K-562), human cervical cancer cell line (He La), and human Burkitt’s lymphoma cell line (Raji), were purchased from the American Type Culture Collection (ATCC, Rockville, MD), while human umbilical veins endothelial cells (HUVECs) were obtained from Cascade Biologics, Inc. Whole plant of Clinacanthus nutans (Burm.f.) Lindau was harvested in November 2011 from botanical garden in Serdang, Selangor, Malaysia. nutans was characterized by the Phytomedicinal Herbarium, Institute of Bioscience, Universiti Putra Malaysia, Selangor (Voucher no. The leaves were then ground into powder and sequentially soaked in chloroform (CNC), methanol (CNM), and distilled water (CNA) at room temperature. The filtrates in chloroform and methanol were dried by using rotary evaporator, while filtrates in distilled water were dried with a freeze drier. DPPH radical scavenging activity of the tested extracts was determined according to the method described by Chan et al. In brief, each extract solution (50 L of DPPH ethanolic solution (0.2 m M).Trolox was used as the standard and DPPH radical scavenging activity of each extract was expressed as L of galvinoxyl radical ethanolic solution (0.125 m M).Then, the mixture was swirled gently for 1 min and kept in the dark for 60 min.Thus, interest has been shifted to study traditional herbs as alternate anticancer regimens due to its multitargeted characteristics.
Hence, we sought to evaluate Clinacanthus nutans Lindau for its potential to be used as natural nutraceuticals for cancer prevention and treatment.
Clinacanthus nutans Lindau (CN), also known as Sabah Snake Grass, from the family Acanthaceae, is a type of famous medicinal plants in Thai folklore medicine.
Previously, CN has traditionally been used to treat inflammation  and viral infection [11, 12].
The extracts in all three solvents were collected separately in clean glass bottles. Then, the mixtures were swirled gently for 1 min and kept in dark for 60 min.
The DPPH radical scavenging activity of each extract was determined by electron spin resonance spectrometry (JEOL-JES-FA100, Tokyo, Japan) using the following parameters: sweeping field, m T; microwave power, 4 m W; modulation width, 0.1 m T; sweep time, 30 s; time constant, 0.1 s; and amplitude, 160.
Clinacanthus nutans Lindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment.